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AFM imaging of RGD presenting synthetic extracellular matrix using gold nanoparticles

Citation:

Hsiong SX, Cooke PH, Kong H-J, Fishman ML, Ericsson M, Mooney DJ. AFM imaging of RGD presenting synthetic extracellular matrix using gold nanoparticles. Macromol Biosci. 2008;8 (6) :469-77.

Date Published:

2008 Jun 11

Abstract:

Several high-resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High-resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin-labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies.
Last updated on 09/29/2017