%0 Journal Article %J Biomaterials %D 2016 %T The effect of surface modification of mesoporous silica micro-rod scaffold on immune cell activation and infiltration %A Li, Weiwei Aileen %A Lu, Beverly Ying %A Gu, Luo %A Choi, Youngjin %A Kim, Jaeyun %A Mooney, David J %K Animals %K Bone Marrow Cells %K Carrier Proteins %K Cytokines %K Dendritic Cells %K Female %K Inflammasomes %K Mice, Inbred C57BL %K NLR Family, Pyrin Domain-Containing 3 Protein %K Porosity %K Silicon Dioxide %K Surface Properties %K Tissue Scaffolds %X Biomaterial scaffold based vaccines show significant potential in generating potent antigen-specific immunity. However, the role of the scaffold surface chemistry in initiating and modulating the immune response is not well understood. In this study, a mesoporous silica micro-rod (MSR) scaffold was modified with PEG, PEG-RGD and PEG-RDG groups. PEG modification significantly enhanced BMDC activation marker up-regulation and IL-1β production in vitro, and innate immune cell infiltration in vivo. PEG-RGD MSRs and PEG-RDG MSRs displayed decreased inflammation compared to PEG MSRs, and the effect was not RGD specific. Finally, the Nlrp3 inflammasome was found to be necessary for MSR stimulated IL-1β production in vitro and played a key role in regulating immune cell infiltration in vivo. These findings suggest that simply modulating the surface chemistry of a scaffold can regulate its immune cell infiltration profile and have implications for the design and development of new material based vaccines. %B Biomaterials %V 83 %P 249-56 %8 2016 Mar %G eng %1 http://www.ncbi.nlm.nih.gov/pubmed/26784009?dopt=Abstract %R 10.1016/j.biomaterials.2016.01.026