%0 Journal Article %J J Biomed Mater Res %D 2000 %T Comparative study of seeding methods for three-dimensional polymeric scaffolds %A Burg, K J %A Holder, W D %A Culberson, CR %A Beiler, RJ %A Greene, K G %A Loebsack, A B %A Roland, W D %A Eiselt, P %A Mooney, D J %A Halberstadt, C R %K Animals %K Biocompatible Materials %K Biomedical Engineering %K Bioreactors %K Cell Count %K Cell Division %K Cells, Cultured %K Endothelium, Vascular %K Materials Testing %K Microscopy, Electron, Scanning %K Polyglycolic Acid %K Rats %X Development of tissue-engineered devices may be enhanced by combining cells with porous absorbable polymeric scaffolds before implantation. The cells are seeded throughout the scaffolds and allowed to proliferate in vitro for a predetermined amount of time. The distribution of cells throughout the porous material is one critical component determining success or failure of the tissue-engineered device. This can influence both the successful integration of the device with the host tissue as well as the development of a vascularized network throughout the entire scaffold volume. This research sought to compare different seeding and proliferation methods to select an ideal method for a polyglycolide/aortic endothelial cell system. Two seeding environments, static and dynamic, and three proliferation environments, static, dynamic, and bioreactor, were analyzed, for a total of six possible methods. The six seeding and proliferation combinations were analyzed following a 1-week total culture time. It was determined that for this specific system, dynamic seeding followed by a dynamic proliferation phase is the least promising method and dynamic seeding followed by a bioreactor proliferation phase is the most promising. %B J Biomed Mater Res %V 51 %P 642-9 %8 2000 Sep 15 %G eng %N 4 %1 http://www.ncbi.nlm.nih.gov/pubmed/10880112?dopt=Abstract