@article {217361,
	title = {Polymeric delivery of proteins and plasmid DNA for tissue engineering and gene therapy},
	journal = {Crit Rev Eukaryot Gene Expr},
	volume = {11},
	number = {1-3},
	year = {2001},
	month = {2001},
	pages = {47-58},
	abstract = {In vivo gene expression can be altered by locally delivered DNA and proteins. The ability to deliver bioactive macromolecules, such as proteins and plasmid DNA, over controllable time frames represents a challenging engineering problem. Considerable success has been achieved with polymeric delivery systems that provide the capability to change cell function either acutely or chronically. This review focuses on controlled delivery of proteins and plasmid DNA from polymers and on the effects of controlled delivery on gene expression, and introduces some cell biological and biochemical parameters to be considered when delivering macromolecules to change cell behavior.},
	keywords = {Animals, Biological Availability, Biopolymers, Delayed-Action Preparations, DNA, Recombinant, Drug Carriers, Drug Compounding, Drug Delivery Systems, Drug Stability, Forecasting, Gene Expression Regulation, Genetic Therapy, Genetic Vectors, Glycolates, Growth Substances, Half-Life, Humans, Lactic Acid, Microspheres, Polyesters, Polyglycolic Acid, Polymers, Proteins, Tissue Engineering},
	issn = {1045-4403},
	author = {Richardson, T P and Murphy, W L and Mooney, D J}
}
