@article {217246,
	title = {Fabrication and in vitro testing of polymeric delivery system for condensed DNA},
	journal = {J Biomed Mater Res A},
	volume = {67},
	number = {4},
	year = {2003},
	month = {2003 Dec 15},
	pages = {1384-92},
	abstract = {Polyethylenimine (PEI) was combined with plasmid DNA and freeze dried following the addition of sucrose as a lyoprotectant and pore-forming agent. Freeze-dried PEI DNA condensates were dry mixed with granular polylactideglycolic acid (PLGA) then compression molded and sponged to encapsulated PEI DNA. A measurement of the elastic modulus indicated that 91 wt\% sucrose substituted for 95 wt\% sodium chloride as a porogen, resulting in PLGA sponges with a mechanical modulus of 100 kPa. The PEI DNA was retained (80\%) within PLGA sponges prepared with sucrose during the leaching and subsequent 2-week release studies, whereas sodium chloride PLGA sponges caused the premature release (100\%) of PEI DNA within 2 days. In vitro gene transfer studies with PEI DNA PLGA sponges established that adherent and infiltrating fibroblasts expressed reporter gene for 15 days compared with the short, 3-day expression mediated by direct gene of PEI DNA on cells in culture. The results demonstrate an approach to encapsulate condensed DNA in a PLGA sponge for the purpose of retaining DNA within the matrices and creating efficient gene transfer during tissue engineering.},
	keywords = {Animals, DNA, Circular, Elasticity, Fibroblasts, Gene Expression Regulation, Gene Transfer Techniques, Genes, Reporter, Lactic Acid, Materials Testing, Mice, NIH 3T3 Cells, Particle Size, Plasmids, Polyethyleneimine, Polyglycolic Acid, Polymers, Sucrose, Surface Properties},
	issn = {1549-3296},
	doi = {10.1002/jbm.a.20036},
	author = {Huang, Yen-Chen and Connell, Maureen and Park, Youmie and Mooney, David J and Rice, Kevin G}
}
