@article {216971,
	title = {Rescue of SCID murine ischemic hindlimbs with pH-modified rhbFGF/poly(DL-lactic-co-glycolic acid) implants},
	journal = {J Control Release},
	volume = {122},
	number = {3},
	year = {2007},
	month = {2007 Oct 08},
	pages = {331-7},
	abstract = {Site-specific controlled release of biologically active angiogenic growth factors such as recombinant human basic fibroblast growth factor (rhbFGF) is a promising approach to improve collateral circulation in patients suffering from ischemic heart disease or peripheral vascular disease. Previously, we demonstrated stabilization of rhbFGF encapsulated in injectable poly(DL-lactic-co-glycolic acid) (PLGA) millicylindrical implants upon co-incorporation of Mg(OH)2 to raise the microclimate pH in the polymer. The purpose of this study was to compare stabilized (S; +Mg(OH)2+other stabilizers), partially stabilized (PS; -Mg(OH)2+other stabilizers), unstabilized (US; no stabilizers), and blank (B) PLGA-encapsulated rhFGF formulations to promote angiogenesis in SCID mice. Following 4 weeks subcutaneous implantation at a 0.1 microg dose in healthy animals, the S group exhibited significantly higher blood vessel density (62+/-17 vessels/mm2) compared with PS, US, and B groups (11+/-2*, 17+/-7*, and 3+/-1** respectively) (* p,
	keywords = {Angiogenesis Inducing Agents, Animals, Disease Models, Animal, Drug Carriers, Fibroblast Growth Factors, Hindlimb, Humans, Hydrogen-Ion Concentration, Ischemia, Lactic Acid, Male, Mice, Mice, SCID, Necrosis, Neovascularization, Physiologic, Polyglycolic Acid, Polymers, Recombinant Proteins, Regional Blood Flow},
	issn = {1873-4995},
	doi = {10.1016/j.jconrel.2007.05.016},
	author = {Zhong, Yanqiang and Zhang, Li and Ding, Amy G and Shenderova, Anna and Zhu, Gaozhong and Pei, Ping and Chen, Ruth R and Mallery, Susan R and Mooney, David J and Schwendeman, Steven P}
}
