@article {1434678,
	title = {An injectable bone marrow-like scaffold enhances T cell immunity after hematopoietic stem cell transplantation},
	journal = {Nat Biotechnol},
	volume = {37},
	number = {3},
	year = {2019},
	month = {2019 03},
	pages = {293-302},
	abstract = {Allogeneic hematopoietic stem cell transplantation (HSCT) is a curative treatment for multiple disorders, but deficiency and dysregulation of T cells limit its utility. Here we report a biomaterial-based scaffold that mimics features of T cell lymphopoiesis in the bone marrow. The bone marrow cryogel (BMC) releases bone morphogenetic protein-2 to recruit stromal cells and presents the Notch ligand Delta-like ligand-4 to facilitate T cell lineage specification of mouse and human hematopoietic progenitor cells. BMCs subcutaneously injected in mice at the time of HSCT enhanced T cell progenitor seeding of the thymus, T cell neogenesis and diversification of the T cell receptor repertoire. Peripheral T cell reconstitution increased ~6-fold in mouse HSCT and ~2-fold in human xenogeneic HSCT. Furthermore, BMCs promoted donor CD4 regulatory T cell generation and improved survival after allogeneic HSCT. In comparison to adoptive transfer of T cell progenitors, BMCs increased donor chimerism, T cell generation and antigen-specific T cell responses to vaccination. BMCs may provide an off-the-shelf approach for enhancing T cell regeneration and mitigating graft-versus-host disease in HSCT.},
	keywords = {Adoptive Transfer, Animals, Bone Marrow, Bone Marrow Transplantation, Chimerism, Graft vs Host Disease, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells, Humans, Mice, T-Lymphocytes, Regulatory, Tissue Scaffolds, Transplantation, Heterologous, Transplantation, Homologous},
	issn = {1546-1696},
	doi = {10.1038/s41587-019-0017-2},
	author = {Shah, Nisarg J and Mao, Angelo S and Shih, Ting-Yu and Kerr, Matthew D and Sharda, Azeem and Raimondo, Theresa M and Weaver, James C and Vrbanac, Vladimir D and Deruaz, Maud and Tager, Andrew M and Mooney, David J and Scadden, David T}
}
