@article {1192451,
	title = {Comparative study of seeding methods for three-dimensional polymeric scaffolds},
	journal = {J Biomed Mater Res},
	volume = {51},
	number = {4},
	year = {2000},
	month = {2000 Sep 15},
	pages = {642-9},
	abstract = {Development of tissue-engineered devices may be enhanced by combining cells with porous absorbable polymeric scaffolds before implantation. The cells are seeded throughout the scaffolds and allowed to proliferate in vitro for a predetermined amount of time. The distribution of cells throughout the porous material is one critical component determining success or failure of the tissue-engineered device. This can influence both the successful integration of the device with the host tissue as well as the development of a vascularized network throughout the entire scaffold volume. This research sought to compare different seeding and proliferation methods to select an ideal method for a polyglycolide/aortic endothelial cell system. Two seeding environments, static and dynamic, and three proliferation environments, static, dynamic, and bioreactor, were analyzed, for a total of six possible methods. The six seeding and proliferation combinations were analyzed following a 1-week total culture time. It was determined that for this specific system, dynamic seeding followed by a dynamic proliferation phase is the least promising method and dynamic seeding followed by a bioreactor proliferation phase is the most promising.},
	keywords = {Animals, Biocompatible Materials, Biomedical Engineering, Bioreactors, Cell Count, Cell Division, Cells, Cultured, Endothelium, Vascular, Materials Testing, Microscopy, Electron, Scanning, Polyglycolic Acid, Rats},
	issn = {0021-9304},
	author = {Burg, K J and Holder, W D and Culberson, CR and Beiler, RJ and Greene, K G and Loebsack, A B and Roland, W D and Eiselt, P and Mooney, D J and Halberstadt, C R}
}
