@article {1192391,
	title = {Effect of substrate mechanics on chondrocyte adhesion to modified alginate surfaces},
	journal = {Arch Biochem Biophys},
	volume = {422},
	number = {2},
	year = {2004},
	month = {2004 Feb 15},
	pages = {161-7},
	abstract = {This study characterized the attachment of chondrocytes to RGD-functionalized alginate by examining the effect of substrate stiffness on cell attachment and morphology. Bovine chondrocytes were added to wells coated with 2\% alginate or RGD-alginate. The alginate was crosslinked with divalent cations ranging from 1.25 to 62.5 mmol/g alginate. Attachment to RGD-alginate was 10-20 times higher than attachment to unmodified alginate and was significantly inhibited by antibodies to integrin subunits alpha3l and beta1, cytochalasin-D, and soluble RGD peptide. The equilibrium level and rate of attachment increased with crosslink density and substrate stiffness. Substrate stiffness also regulated chondrocyte morphology, which changed from a rounded shape with nebulous actin on weaker substrates to a predominantly flat morphology with actin stress fibers on stiffer substrates. The dependence of attachment on integrins and substrate stiffness suggests that chondrocyte integrins may play a role in sensing the mechanical properties of the matrices to which they are attached.},
	keywords = {Alginates, Amino Acid Sequence, Animals, Barium, Biocompatible Materials, Calcium, Cattle, Cell Adhesion, Chondrocytes, Cross-Linking Reagents, Cytochalasin D, Dose-Response Relationship, Drug, Kinetics, Microscopy, Electron, Scanning, Oligopeptides, Surface Properties},
	issn = {0003-9861},
	doi = {10.1016/j.abb.2003.11.023},
	author = {Genes, Nicholas G and Rowley, Jonathan A and Mooney, David J and Bonassar, Lawrence J}
}
