@article {1192206,
	title = {In vivo time-gated fluorescence imaging with biodegradable luminescent porous silicon nanoparticles},
	journal = {Nat Commun},
	volume = {4},
	year = {2013},
	month = {2013},
	pages = {2326},
	abstract = {Fluorescence imaging is one of the most versatile and widely used visualization methods in biomedical research. However, tissue autofluorescence is a major obstacle confounding interpretation of in vivo fluorescence images. The unusually long emission lifetime (5-13 μs) of photoluminescent porous silicon nanoparticles can allow the time-gated imaging of tissues in vivo, completely eliminating shorter-lived (50-fold in vitro and by >20-fold in vivo when imaging porous silicon nanoparticles. Time-gated imaging of porous silicon nanoparticles accumulated in a human ovarian cancer xenograft following intravenous injection is demonstrated in a live mouse. The potential for multiplexing of images in the time domain by using separate porous silicon nanoparticles engineered with different excited state lifetimes is discussed.},
	keywords = {Animals, Female, Humans, Luminescent Agents, Mice, Nanoparticles, Neoplasm Transplantation, Optical Imaging, Ovarian Neoplasms, Silicon, Xenograft Model Antitumor Assays},
	issn = {2041-1723},
	doi = {10.1038/ncomms3326},
	author = {Gu, Luo and Hall, David J and Qin, Zhengtao and Anglin, Emily and Joo, Jinmyoung and Mooney, David J and Howell, Stephen B and Sailor, Michael J}
}
