@article {1092276,
	title = {In Vivo Enrichment of Diabetogenic T Cells},
	journal = {Diabetes},
	volume = {66},
	number = {8},
	year = {2017},
	month = {2017 Aug},
	pages = {2220-2229},
	abstract = {Dysfunctional T cells can mediate autoimmunity, but the inaccessibility of autoimmune tissues and the rarity of autoimmune T cells in the blood hinder their study. We describe a method to enrich and harvest autoimmune T cells in vivo by using a biomaterial scaffold loaded with protein antigens. In model antigen systems, we found that antigen-specific T cells become enriched within scaffolds containing their cognate antigens. When scaffolds containing lysates from an insulin-producing β-cell line were implanted subcutaneously in autoimmune diabetes-prone NOD mice, β-cell-reactive T cells homed to these scaffolds and became enriched. These T cells induced diabetes after adoptive transfer, indicating their pathogenicity. Furthermore, T-cell receptor (TCR) sequencing identified many expanded TCRs within the β-cell scaffolds that were also expanded within the pancreata of NOD mice. These data demonstrate the utility of biomaterial scaffolds loaded with disease-specific antigens to identify and study rare, therapeutically important T cells.},
	keywords = {Adoptive Transfer, Animals, Antigens, Autoimmunity, Cell Line, Cell Movement, Diabetes Mellitus, Type 1, Female, Insulin-Secreting Cells, Mice, Mice, Inbred C57BL, Mice, Inbred NOD, Pancreas, Receptors, Antigen, T-Cell, T-Lymphocytes, Tissue Scaffolds},
	issn = {1939-327X},
	doi = {10.2337/db16-0946},
	author = {Thelin, Martin A and Kissler, Stephan and Vigneault, Frederic and Watters, Alexander L and White, Des and Koshy, Sandeep T and Vermillion, Sarah A and Mooney, David J and Serwold, Thomas and Ali, Omar A}
}
